Fluorometric liquid chromatographic determination of aliphatic aldehydes arising from lipid peroxides.
نویسندگان
چکیده
منابع مشابه
Fluorometric liquid-chromatographic determination of serum cortisol.
We describe a sensitive and specific liquid-chromatographic assay for serum cortisol, exploiting acid-induced fluorescence. Each analysis requires only 50 microL of serum, and chromatography is complete in 7 min. Analytical recovery of cortisol added to serum ranged from 90 to 105% and between-run precision (CV) from 6 to 8%. The lower limit of detection for cortisol is 10 micrograms/L, and lin...
متن کاملFluorometric Determination 1 , 3 - biphenyl - 2 - thiobarbituric of Lipid Acid Peroxides in Tissues with
Lipid peroxides in tissues were fluorometrically determined using 1,3-diphenyl-2-thiobarbituric acid (DPTBA) as a fluorogenic reagent, and the results were compared with those obtained by the DPTBA-visible method, thiobarbituric acid (TBA)-fluorescence method, or TBA-visible method. The optimum conditions for determining lipid peroxides were analyzed by using brain homogenate. The optimum pH fo...
متن کاملGas-liquid chromatographic determination
A method for the determination of total bile acids in human feces that is suitable for routine application is described and discussed. Bile acids are extracted from freezedried feces with acetic acid and toluene, in the presence of the i?ternal standard 23-nordeoxycholic acid. After saponification of the extract, bile acids and the internal standard are methylated and converted by mild chromic ...
متن کاملLiquid-chromatographic determination of vitamin K1 in plasma, with fluorometric detection.
This assay for phylloquinone (vitamin K1) in plasma requires a single liquid-chromatographic step. Much smaller volumes of plasma (0.5-1.0 mL) are required than in previous assays. Before liquid chromatography, we purified crude lipid extracts by conventional chromatography on silica, then extracted the lipid fraction by dissolving it in an acidic mixture of hexane/acetonitrile (1/4 by vol) con...
متن کاملHIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF THEOPHYLLINE IN HUMAN SERUM
A simple, rapid and sensitive HPLC method for the determination of theophylline (T) in human serum has been developed. An isocratic system consisting of a /-l Bondapak C18 column, mobile phase of methanol, phosphate buffer (22:78, pH=4.5), and a flow rate of 1.4 mL/min was used. The eluent was detected by UV at 275 nm at room temperature. 8-Chlorotheophylline (8-CT) was used as an internal ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1986
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.34.1694